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F., Sheffy, J., Vita, J

The classic pattern here is high fasting insulin, high LDL cholesterol, low HDL, and high triglycerides

Immunoblot analysis Total protein was extracted from frozen tissue (100 mg) in 500 L RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF, 1 protease inhibitor cocktail) on ice for 30 min, cleared by centrifugation (12 000 g, 15 min, 4 C), and quantified with the BCA assay

Continue until all of the truffle mixture is used up

DOI: 10.7554/eLife.00007 Danny Kessler

These ranged from unusual reputational issues, including retrieving container-loads of cigarettes sold to Iraq in breach of UN sanctions and obscuring the fact that a BAT finance director, who died in a Nairobi hotel room, had passed away after a drug-fuelled orgy with two prostitutes