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Description
For the HWP1 , the thermocycling conditions were 3 min at 95C, followed by 30 cycles of 10 s at 95C, 30 s at 54C, and 40 s at 72C, with each reaction also performed in triplicate
All of these are hand made, one at a time
You should not travel if it prevents you from fulfilling these obligations
Nature 415: 9699, 2002
Intern Emerg Med 16:22012212
Wire guage is 16AWG
