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Potential mechanisms for this interaction include (1) cooperation between germline and somatic variants in different pathways to promote a clonal advantage 318 , (2) germline variants acting downstream of somatic variants to modulate clonal advantage (and vice versa), (3) germline variants promoting the formation of larger-scale somatic events such as chromosomal abnormalities 177 , and (4) germline variants (for example, in DNA repair pathways) contributing to clonal advantage, agnostic to the nature of the somatic mutation driving the clone

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The real reason that phosphates have to be introduced into the soil is that the tobacco plant is a very soil depleting plant and requires the addition of phosphates, potassium, and nitrates into the soil

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