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In 2008, with free agency looming on the horizon and very little hope they'd be able to re-sign Sabathia in the offseason, the Indians dealt the flame-throwing lefty to the Milwaukee Brewers for blue-chip prospect Matt LaPorta and a trio of other minor leaguers

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After incubation, the coverslip containing the cells were washed with PBS, fixed in Paraformaldehyde 4% for 15 min RT, then washed in PBS and permeabilized for 5 min with Buffer (0.05% Saponin in PBS- plus 3% BSA) and then incubated with blocking Solution (BSA 0,5% in PBS) 1 h RT

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