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[10] Widespread adoption of this type of policy can also impact prices and availability in places without the policy in place

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230 Backed by influential NGOs like Health Justice and the Bloomberg-supported Southeast Asia Tobacco Control Alliance (SEATCA), health officials have consistently warned the public against the adverse health effects and safety concerns associated with the use of e-cigarettes. According to the DOH, e-cigarettes contain harmful chemicalssuch as nicotine, ultra-fine particles, carcinogens, heavy metals, and volatile organic compounds. The agency cited peer-reviewed studies, which showed that e-cig juices contain high levels of addictive nicotine, which can result in acute or even fatal poisoning through ingestion and other means. The DOH claimed that there are documented cases of nicotine toxicity in children of epidemic proportion in other countries with increasing prevalence of e-cigarette use and that vapour contains harmful substances that can affect bystanders

For DNA degradation, the purified DNA was subjected to Nuclease P1 treatment (Sigma, N8630) at 37 C for 4 h

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