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Background Renin-angiotensin system inhibitors (RASi) and sodium glucose cotransporter inhibitors (SGLT2i) are known for their kidney protective properties, but both show significant residual risk in large outcome trials

Injection pain during GLP-1 therapy often stems from technique, needle depth, or skin temperaturenot the medication itself

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We subsequently developed an in vivo technique to assess gut hormone release into the portal vein following colonic infusion of propionate