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Further, the -helix fractions ( f a ) of SR18 calculated at 222 were found to be in the range of 0.034 in 1X PBS, 0.12 in 20% TFE, 0.22 in 20% HFIP, and 0.17 in 30 mM SDS (Supplementary Table S2), which is noted to be comparable with the reported value of 0.027 for semaglutide ( FIGURE 9 Further, Dynamic Light Scattering (DLS), a widely used technique for determining the hydrodynamic size and size distribution of molecules in a sample by analyzing fluctuations in scattered light due to Brownian motion, was used to evaluate the hydrodynamic size distribution of 100 M SR18 under different solvent conditions