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For histology, the left hepatic lobe was enclosed in a prelabelled histology cassette and fixed in 10% neutral buffered formalin for 24 h, followed by 70% ethanol for another 24 h before submission for tissue processing

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Cells were cultured at 37 C in Dulbeccos modified Eagles medium (DMEM) + GlutaMax 1965 (Gibco) supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin in a humidified 10% CO 2 incubator

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