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Cultures were prepared from embryos (E15.5) of CD-1 mice (Charles River Laboratories)

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PCR results were analyzed as threshold cycle (C T ) values to determine the relative amounts of target gene mRNA compared to the reference gene mRNA, with Gapdh used as the internal reference gene

Forbes, J

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The 1.7mg dose was specifically studied in the STEP clinical trials as an intermediate maintenance option for people who experienced intolerable side effects at 2.4mg but wanted more effect than 1.0mg provided