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All patients were given a subcutaneous injection of GLP-1 and an intravenous glucose bolus

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Adding once-daily lixisenatide for type 2 diabetes inadequately controlled with newly initiated and continuously titrated basal insulin glargine: a 24-week, randomized, placebo-controlled study (GetGoal-Duo 1)

We denote this end of the trajectory as the most like. The -3, -4, and -5 subclusters were at the other end of the pseudotime trajectory, exhibiting the highest expression of MAFA and PDX1 , suggesting that these subpopulations were the most mature cell subpopulations

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This is a real consideration, not a minor footnote