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Ex vivo glucose-stimulated insulin secretion (GSIS) in pancreatic islets After G49 treatment, pancreatic islets were incubated in KRB with 2.8 mM glucose and 0.25% BSA, pH 7.4 for 1 h at 37 C in an atmosphere of 5% CO 2

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Additionally, obesity and IR both contribute to -cell dysfunction and apoptosis, mechanistically explained by glucotoxicity and increased immunogenicity (the accelerator hypothesis) or by -cell exhaustion due to increased metabolic demand (the overload hypothesis) [23]

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