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doi: 10.1136/jnnp.71.1.1

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The entire intestine was removed, rinsed, and chopped into 12 mm pieces in ice-cold Ca 2+ -free Hanks balanced salt solution containing 0.65 mM dithiothreitol, 1% BSA, penicillin, streptomycin, 9.05 mM N a HCO 3 , and 20 mM HEPES

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