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doi: 10.1038/ncomms8629 [DOI] [PMC free article] [PubMed] [Google Scholar] 41
10.1056/NEJMoa2301972
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Twenty-four hours after transfection, cells were lysed using 1 mL of TNTE lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 5 mM EDTA, and 0.3% Triton X-100) supplemented with 1 PhosSTOP (Roche) and 1 EDTA-free Complete Protease Inhibitor Cocktail (Roche)
