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Previous studies have indicated that separate treat- ment of hypertension with lisinopril partially reverses albuminuria and glomerulosclerosis in db/db UNx-ReninAAV mice [ 13 , 14 , 31 ]

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Upon reaching the 1-day adult stage, 30 worms were transferred to each of three RNAi plates and their status was monitored daily or every other day by counting the number of live or dead worms

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