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3.5 Semaglutide intervention and fecal microbiota transplantation improved the dysbiosis of gut microbiota in obese mice, increasing the relative abundance of beneficial bacteria, and affecting the levels of short-chain fatty acids The species accumulation curve and dilution curve indicated that the sample size and sequencing depth were sufficient (Supplementary Figure S3A)

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In this study, we established a procedure to isolate and culture cells derived from murine Brunner's glands

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