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The measurement volume was calibrated on each experimental day with 20 nM Rhodamine 6 G (Diffusion Coefficient, D, 2.8 x10 -10 m 2 s -1 ) in HPLC grade water

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results are aligned with ours revealing that BMI reductions that were seen (1.147 kg/m 2 at 24 months) indicate that GLP-1 RA weight loss effects contribute to therapeutic advantages by reducing intracranial venous pressure and decreasing the generation of pro-inflammatory adipokines

Additionally, GLP-1 RAs may reduce -cell apoptosis, further contributing to the risk of neoplastic transformation [27]

For instance, in 3xTg-AD mice, sex-specific alterations in brain bioenergetics have been observed: females showed impaired complex I activity in synaptic cortical mitochondria, while non-synaptic mitochondria exhibited enhanced complex IImediated respiration (Stojakovic et al., 2021)

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