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The cultured cells were grouped as follows: NT, LPS (1 g/mL), GLP-1 (50 mol/L), LPS (1 g/mL) + GLP-1 (10, 20, and 50 mol/L) groups

This simple step prevents the introduction of bacteria and other contaminants
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Brains were then snap-frozen in isopentane and the OB was cut into 18 m-thick sections afterwards, using a cryostat (Leica Biosystems)
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