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The tissue was then digested for 15 min in a 37 C shaking water bath incubator using 5 ml of 100 U/ml collagenase type I (Sigma) in Basal Medium Eagle (Thermo Fisher Scientific) and supplemented with 1% BSA, 26.4 mM N a HCO 3 , and 10 mM HEPES (pH 6.9)

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Hemoglobin is the protein in red blood cells that sticks to oxygen so it can be ferried around the body