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Given the low circulating and tissue levels of most DPP-4 substrates and the technical challenge inherent in quantifying intact versus cleaved peptides, it seems likely that some DPP-4 substrates currently classified as pharmacological may one day also be found to be cleaved by DPP-4 in vivo [20]

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However, native GLP-1 has limited pharmacological value because of its short half-life (12 min), attributable to degradation by the peptidase enzyme DPP-4 10

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