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Fifty nanograms of vector with 3-fold molar excess of insert were ligated using the Quick Ligation Kit (New England Biolabs)

Rosenstock J, Wysham C, Fras JP, Kaneko S, Lee CJ, Fernndez Land L, Mao H, Cui X, Karanikas CA, Thieu VT

Reconstituted tirzepatide must be stored in the refrigerator at 36 to 46 degrees Fahrenheit (2 to 8 degrees Celsius)

Although its not yet confirmed if this risk applies to humans, individuals with a personal or family history of MTC or Multiple Endocrine Neoplasia syndrome type 2 should avoid using tirzepatide

Triage safety using the contraindications and red flag screens, then route the file to provider review

Han et al., 2018a