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To generate this data set, we applied DART-Seq, a scRNA-Seq technique, to the study of human islets

GLP-1 Diet Plan: What to Eat on Ozempic, Wegovy or Mounjaro Updated April 20, 2026, Medical Review by Kaitlyn Wong, RDN If you are on a GLP-1 medication like Ozempic, Wegovy, Zepbound, or Mounjaro, your appetite and digestion can change fast

She explained that she had been dealing with a thyroid issue for many years that can affect metabolism and contribute to weight fluctuations

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In studies where patients tapered off slowly, hunger hormones rose more gently, and weight regain was smaller

Sample sections were blocked in 5 % goat serum with 0.3 % Triton X-100 for 1 h at RT and then incubated overnight at 4 C with the following primary antibodies: mouse anti-GLP-1R (1:50, Santa Cruz, CA, USA), mouse anti-GLP-1(1:50, Santa Cruz, CA, USA), rabbit anti-Iba1 (1:500, Wako Chemicals, Tokyo, Japan), rabbit anti-GFAP (1:200, Cell Signalling, Boston, MA, USA), rabbit anti-NeuN (1:100, Proteintech, China), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), and mouse anti-c-fos (1:100, Santa Cruz, CA, USA)