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Gene expression levels were quantified by qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA) and reactions were performed on a Viia TM 7 real time PCR system (Life Technologies, Gaithersburg, MD, USA)

This mechanism promotes caloric deficit by decreasing appetite drive rather than forcing restriction through willpower alone

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doi: 10.2337/dc17-1956 [DOI] [PubMed] [Google Scholar] 58

D., Bluher, M., Tschop, M

This information is clear on the website, but many patients miss that the first month is discounted and medication is extra