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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants

Wen S, Nguyen T, Gong M, Yuan X, Wang C, Jin J, et al

Powerlifting, bodybuilding, and physique sports have their own federation-specific anti-doping rules that vary considerably

And consider leveraging specialized services if accessible

doi:10.2337/dc19-0415 He L, Wang J, Ping F, Yang N, Huang J, Li Y, Xu L, Li W, Zhang H

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