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The most impressive structural feature of peptide 20 is the lipidated K10 P by a 16-carbon palmitic acid through a -carboxylate spacer, which perfectly inserted into the TM1-TM2 crevice and made extensive contacts with both receptors and lipid membrane to stabilize the binding poses (Fig

Cells were cultured at 37 C in Dulbeccos modified Eagles medium (DMEM) + GlutaMax 1965 (Gibco) supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin in a humidified 10% CO 2 incubator

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And, exendin-4 significantly increased A-induced decrease in the level of phosphorylated Akt in brain (189)

doi: 10.1111/j.1572-0241.2004.21436.x