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After three washes in TBS-T, the membranes were incubated with corresponding secondary antibodies, with goat anti-rabbit IgG-h + HRP conjugated (Bethyl cat# A120-101p) and donkey anti-mouse IgG-h-I HRP conjugated (Bethyl cat# A90-137p) (1:5000) secondary antibodies, for 1 h at room temperature
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Weight loss accelerates during this phase, with typical losses of 2-4 pounds per week
By attenuating the subjective effects and reinforcing effects of alcohol, as supported by the present data and previous work, respectively (Egecioglu et al
