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The antibodies used in WB were: INSULIN (1:1000, ab181547, Abcam), PPAR (1:1000, 74076 S, CST), UCP2 (1:1000, 89326 S, CST), PDX1 (1:1000, ab219207, Abcam), MAFA (1:1000, 7937 S, CST), IRS1 (1:1000, 2390 S, CST), GCK (1:1000, 19666-1-AP, Proteintech), -ACTIN (1:1000, PM053, MBL) and -TUBULIN (1:1000, 2128 S, CST)

Another possibility of the direct effects of GLP-1 and ghrelin in the brain has been proposed since both peptides cross the brain blood barrier 25,35

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