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Human endogenous GLP-1 cleaves GLP-1 (7-36 amide) and GLP-1 (7-37) on the N-terminal dipeptide very quickly in the presence of dipeptidyl peptidase 4 (DPP-4), generating low-affinity ligands of the GLP-1 receptor, GLP-1 (9-36 amide) or GLP-1 (9-37), which gives it a half-life of only 1.5 min2 min (Deacon et al., 1995
For a researcher, that open question is half the appeal, because it is exactly the kind of thing a well-designed in-vitro comparison can probe
