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Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

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serotonin) signalling is likely to influence nucleus tractus solitarius GLP1 neuronal activity with resulting actions on metabolism (D'Agostino et al., 2018