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Identity verification via mass spectrometry confirms correct molecular weight of 4113.58 g/mol

The gyration radius reached the plateau after 130 ns and remained constant over the timeframe with an average value of 2.32, 2.73, and 2.7 nm for nGLP1-DARPin, mGLP1-DARPin-1, and mGLP1-DARPin-2, respectively, which signifies that all systems remained compact across the 500 ns MD simulation (Fig

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