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( A ) Intracellular accumulation of cAMP was measured by enzymatic immunoassay in lysates from caSMCs treated for 10 minutes with 100 nM each of GLP-1, forskolin, or PBS control, with or without 20 M sacubitril, a neutral endopeptidase inhibitor ( n = 3/treatment, each in triplicate)

However, in chloralose-anaesthetised pigs, electrical stimulation of the vagal trunks at the level of the diaphragm had no effect on GLP-1 secretion, and human cephalic phase studies and studies in vagotomised humans found similar results 2830

These foods, which are typically low in nutritional value but high in simple carbohydrates and unhealthy fats, exacerbates IR and promotes visceral fat accumulation, a key risk factor for T2DM [37]

doi: 10.1016/j.eclinm.2023.101865 23 KlausenMKThomsenMWortweinGFink-JensenA

While DPP-4 expression is significantly increased in patients with obesity and T2DM, the incretin effect is markedly impaired
