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Possible Long-Term Efficacy of Sitagliptin, a Dipeptidyl Peptidase-4 Inhibitor, for Slowly Progressive Type 1 Diabetes (SPIDDM) in the Stage of Non-Insulin-Dependency: An Open-Label Randomized Controlled Pilot Trial (SPAN-S)

a , Mitochondrial oxygen consumption rate (OCR) during mitochondrial stress test of healthy primary murine hepatocytes treated ex vivo for 4h with 100 nM cotadutide, g1437 or liraglutide compared to vehicle control hepatocytes (n=3 biologically independent samples/group)

Proc Natl Acad Sci U S A 96:4586

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Clear solution This protocol yields a clear solution of 2.5 mg/mL (saturation unknown)

DNA was finally recovered with the commercial QIAamp DNA Fast Stool Mini Kit (QIAGEN GmbH, Hilden, Germany), following the manufacturers instructions and quantified using Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA)