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How Semaglutide Stability Works: Temperature, Time, and Degradation Semaglutide is a peptide hormone sensitive to temperature fluctuations and light exposure

After loading, matrix washed with same urea buffer and started eluting the impurities with elution buffer containing 20mM Imidazole followed by 250mM Imidazole to give elute having pure His6-AV- Ubiquitin-semapeptide