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A midline incision was made to provide wide exposure of the upper abdominal organ in male C57BL/6J mice anesthetized with tribromoethanol (200 mg kg 1 , i.p.)

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doi: 10.1016/S2213-8587(19)30180-9

Moreover, the elevated markers of oxidative stress precede A deposition and NFTs, suggesting that oxidative stress is an early event in the pathogenesis of AD (67)