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Thermo Fisher Scientific, Inc.) using SYBR Premix Ex Taq II (Takara Bio, Inc.) The PCR protocol comprised three stages: Initial denaturation at 95C for 30 sec to activate the DNA polymerase and denature templates

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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Effects of testosterone treatment in older men

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A BMI of 27 or higher if you're also dealing with at least one weight-related health issue, like high blood pressure, type 2 diabetes, or high cholesterol