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The proglucagon gene encodes a 180-amino acid precursor that, depending on the tissue-specific expression of processing enzymes, yields different active peptides: in the pancreatic alpha cells, PCSK2 processing produces glucagon

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For the second experiment (Fasting study): Mice were sacrificed after 16 h of fasting, and livers were collected and snap-frozen in liquid nitrogen until further processing

Marso SP, Bain SC, Consoli A, Eliaschewitz FG, Jdar E, Leiter LA, Lingvay I, Rosenstock J, Seufert J, Warren ML, Woo V, Hansen O, Holst AG, Pettersson J, Vilsbll T

Pathogenic mechanisms of metabolic dysfunction-associated steatotic liver disease (MASLD)-associated hepatocellular carcinoma