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This binding mode was elucidated through cryo-electron microscopy studies published in the Proceedings of the National Academy of Sciences in 2020, revealing that a small molecule could stabilize the active conformation of a class B G protein-coupled receptor (GPCR) through an entirely novel mechanism

GIP signaling further upregulates the expression of the POMC gene in these neurons and amplifies their intracellular cAMP/protein kinase A (PKA) signaling pathway, thereby enhancing the production of satiety signals

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Yet protein purification and sequencing is a lengthy processbiochemical isolations from gut tissue also yield relatively small amounts of protein, making it difficult to study the function of these molecules in the lab