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For each amplification cycle, a threshold cycle (C t ) value was obtained, and C t was calculated as the C t difference between target mRNA and housekeeping mRNA (-Actin)

The CoM patent on the CAR construct is important, but the commercially decisive IP is the manufacturing process itself the specific lentiviral or retroviral vector design, the transduction efficiency optimization, the T cell expansion protocol, the cryopreservation process, and the release assay specifications that determine product quality and clinical consistency

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