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Prior to treatment, medium was replaced and supplemented with 0.1% BSA, and cells were incubated at 37C overnight

The most commonly reported adverse event across all groups was GI distress (nausea, constipation, abdominal pain, diarrhea)
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To further elucidate which GLP-1R-expressing cells are involved in the abovementioned cardio- and vasoprotective benefits, liraglutide was administered to wild-type (C57BL/6J), global Glp1r knockout ( Glp1r / ), endothelial cell- ( Cdh5 Cre ) and myeloid cell ( LysM Cre )-specific knockout of Glp1r and arterial hypertension was induced by angiotensin II

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doi: 10.3389/fimmu.2017.01107 52 da SilvaESFeresMFigueiredoLCShibliJARamiroFSFaveriM
