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Raster Image Cross-Correlation Spectroscopy (RICCS) with Number and Brightness (N&B) image acquisition and data processing INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto -Slide 8-well glass bottom microscope plates (Ibidi) and labelled with SNAP-Surface Alexa Fluor 647 for 10 minutes followed by Laurdan (6-dodecanoyl-2-(dimethylamino) naphthalene), an environmentally sensitive plasma membrane lipid dye (34), for 5 minutes prior to imaging