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Briefly, PWA mice were individually placed in the metabolic cages 24 h prior to initiation of the experiment to allow for an adaptation period

KarsdalMAHenriksenKBay-JensenACMolloyBArnoldMJohnMRet al

The nuclei pellet was resuspended in 2% BSA in PBS supplemented with Protector RNase inhibitor (03335402001, Sigma-Aldrich) at 0.2 U/ul