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Although the substitution of C236 3.39b appeared to have minimal effects on GLP-1 pharmacology, the mutation of the conserved C226 3.29b in two studies led to either no detectable activity or a 25-fold reduction in GLP-1 affinity with 38-fold lower potency

References Yang, B., Good, D., Mosaiab, T., Liu, W., Ni, G., Kaur, J., Liu, X., Jessop, C., Yang, L., Fadhil, R., Yi, Z., & Wei, M

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