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Example 21 preparation of Semetreuptade 58.3 g of the peptide resin obtained in example 17 were charged into a 2000ml single-neck flask, and lysate 600ml of TFA was prepared in advance: and (3) TIS: EDT (electro-thermal transfer coating): PhOH: H2O 90:3:3:2:2 (vol.%), the lysate was added to the flask, the reaction was carried out at room temperature for 2.5 hours, the resin was filtered off, the resin was washed with 50ml tfa, the filtrates were combined, 6000ml of anhydrous ether was added to precipitate a white solid, the solid was centrifuged, the solid was washed with anhydrous ether, and 19.98 g of crude semaglutide peptide was dried in vacuo to a white solid, with a yield of 98.41%