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For recycling, cells were incubated with agonists at 37 C for 15 min, followed by endocytosis arrest at 4 C, surface label removal as above, 30 min incubation at 37 C with 10 M exendin(9-39) to prevent further internalization, and a second round of surface label removal at 4 C

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Sanetra AM, Palus-Chramiec K, Chrobok L, Jeczmien-Lazur JS, Klich JD, Lewandowski MH

Mechanism: Why these drugs work (and why they cause problems) Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) were initially approved in 2005 for the treatment of type 2 diabetes

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