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For size fractionation studies, bacterial cell-free supernatants were separated by size using centrifugal filter units (Amicon)

The cells were analyzed with the Seahorse XFe24/96 Extracellular Flux analyzer (Agilent) to determine real-time oxygen consumption rate and extracellular acidification rate and normalized the Seahorse measurements by counting cell number with Biotek citation 1/5, as previous reported 7

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