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Three weeks later, mice were terminally anaesthetised with 100 mg/kg pentobarbital and transcardially perfused with PBS containing 4% PFA, brains were collected, post-fixed in 4% PFA at 4 C overnight and cryoprotected in 30% sucrose for 23 days

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doi: 10.1074/jbc.M500540200 [DOI] [PubMed] [Google Scholar] 115
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Peak plasma concentration occurs 1-3 days post-injection, with gradual decline over the following 4-6 days
