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The sections were visualized using 3,3-diaminobenzidine (Vector Laboratories, Burlingame, CA) at room temperature (RT) for 1.5 min and counterstained with hematoxylin
With the city nearly built out, many residents want what is left to be conserved

This is one reason weight maintenance is often harder than weight loss

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Its appeal also comes from its convenience: a once-weekly injection is preferable to drugs that need to be taken once or even several times a day