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Clin Neurophysiol 125(9):19061911 Hanyu N, Oguchi K, Yanagisawa N, Tsukagoshi H (1982) Degeneration and regeneration of ventral root motor fibers in amyotrophic lateral sclerosis: morphometric studies of cervical ventral roots
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More than 9000 patients were included with follow up of 3.8 years
Stimulation buffer was then decanted, and cAMP lysis buffer (50 mM HEPES, 10 mM CaCl 2 , 0.35% Triton X-100, and 500 M IBMX) was added and shaken at 700 rpm for 1 h
