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The RNA was then purified using the RNeasy Mini Kit (Qiagen, 74106) (for whole worm samples) and Zymo RNA Clean-up kit (Zymo Research, Cat

Resistance training and high protein intake of 1.2-1.6 g/kg/day recommended to minimize muscle loss, PMID: 40609566) Mild-to-moderate cardiac arrhythmias reported in some participants long-term cardiovascular outcome data pending from the 113-week TRIUMPH-3 trial (PMID: 37902090) Dose-dependent GI side effect profile adverse event rates roughly doubled between 1 mg and 8 mg groups in Phase 2, making gradual titration critical Phase 2 discontinuation rate for adverse events: 5-10% at highest doses comparable to discontinuation rates seen with semaglutide and tirzepatide Potential blood glucose effects in non-diabetic individuals due to glucagon receptor activation the glucagon component raises and the GLP-1/GIP components lower blood glucose, creating complex interactions under investigation in Phase 3 Long-term safety data beyond 68 weeks not yet available the 113-week TRIUMPH-3 trial in cardiovascular disease patients will provide the longest safety dataset when results are reported Dosing protocol Typical Dose 1 mg to 12 mg weekly (Phase 2/3 doses) Frequency Once weekly subcutaneous injection Not yet FDA-approved all dosing information reflects clinical trial protocols, not prescribing guidance

However, these findings are derived from theoretical physiological models [99] and remain hypothetical

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