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Cell viability was assessed using CCK8 assay (K1018, APEBio, Houston, USA) according to the manufacturers instructions by measuring optical density at 450 nm

If you suspect sleep apnea (snoring, gasping, daytime fatigue despite adequate time in bed), a sleep study can identify treatable conditions that undermine both weight loss and overall health

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This combination reduces the skin barrier's ability to retain moisture, creating that tight, dry sensationparticularly noticeable on the face, hands, and elbows