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A total number of 146 mice were used in this study

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These findings should therefore be interpreted as exploratory and hypothesis-generating rather than confirmatory evidence of a direct reproductive effect independent of weight reduction (102)

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Metformin increases levels of growth differentiation factor 15 (GDF15) in the kidney and circulation to activate glial cell line-derived neurotrophic factor family receptor -like (GFRAL) in the area postrema to reduce body weight
